Journal: Molecular & Cellular Proteomics : MCP

Article Title: Host Plants Indirectly Influence Plant Virus Transmission by Altering Gut Cysteine Protease Activity of Aphid Vectors *

doi: 10.1074/mcp.M116.063495

Figure Lengend Snippet: CathB enzymatic activity assays on turnip-reared (T-Myzus) and physalis-reared (P-Myzus) Myzus persicae, before and after treatment with the cysteine protease inhibitor E-64.

Article Snippet: Cathepsin Inhibition and PLRV Transmission Assays Adult aphids were placed in dishes covered by a Parafilm® sandwich membrane containing an aphid diet prepared with balanced amounts of amino acids ( 45 ) for 48 h. The adults were then removed, and first instar nymphs were transferred to a fresh diet containing the cysteine protease inhibitor E-64 (AG Scientific) at 0, 10, 30, or 50 μ m , for a 48h AAP.

Techniques: Activity Assay, Protease Inhibitor

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Host Plants Indirectly Influence Plant Virus Transmission by Altering Gut Cysteine Protease Activity of Aphid Vectors *

doi: 10.1074/mcp.M116.063495

Figure Lengend Snippet: Cathepsin inhibition modulates PLRV transmission by Myzus persicae. Proportion of plants infected with PLRV by aphids reared on turnip (T-Myzus) A) and on physalis (P-Myzus) B) after feeding on different concentrations of the cysteine protease inhibitor E-64.

Article Snippet: Cathepsin Inhibition and PLRV Transmission Assays Adult aphids were placed in dishes covered by a Parafilm® sandwich membrane containing an aphid diet prepared with balanced amounts of amino acids ( 45 ) for 48 h. The adults were then removed, and first instar nymphs were transferred to a fresh diet containing the cysteine protease inhibitor E-64 (AG Scientific) at 0, 10, 30, or 50 μ m , for a 48h AAP.

Techniques: Inhibition, Transmission Assay, Infection, Protease Inhibitor

Journal: Cell Host & Microbe

Article Title: A Replication-Competent Vesicular Stomatitis Virus for Studies of SARS-CoV-2 Spike-Mediated Cell Entry and Its Inhibition

doi: 10.1016/j.chom.2020.06.020

Figure Lengend Snippet: rVSV-SARS-CoV-2 S Infection Requires the Activity of Cysteine Cathepsin Proteases (A) Huh7.5.1 cells pre-treated for 1 h at 37°C with the indicated concentrations of NH 4 Cl were infected with pre-titrated amounts of rVSVs bearing SARS-CoV-2 S or EBOV GP. Infection was scored by eGFP expression at 16–18 h post-infection (average ± SD, n = 8 from 2 independent experiments). (B) Vero cells pre-treated for 90 min at 37°C with the indicated concentrations of pan-cysteine cathepsin inhibitor E-64 were infected with pre-titrated amounts of rVSVs bearing SARS-CoV-2 S, EBOV GP, or VSV G and scored for infection as above (average ± SD, n = 6 from 3 independent experiments, except n = 4 from 2 independent experiments for EBOV GP). (C) Vero cells pre-treated for 90 min at 37°C with the indicated concentrations of cathepsin L/B inhibitor FYdmk were infected with pre-titrated amounts of rVSVs bearing SARS-CoV-2 S, EBOV GP, or VSV G. Infection was scored as above (average ± SD, n = 6 from 3 independent experiments). (D) Vero cells and Vero cells overexpressing TMPRSS2 pre-treated for 120min at 37C with the indicated concentrations of camostat ]were infected with pre-titrated amounts of rVSVs bearing SARS-CoV-2 S and subsequently scored for infection. In panels (B)–(D), all comparisons were made between vehicle- and inhibitor-treated samples. ns,not statistically significant. ∗ p < 0.033, ∗∗∗ p < 0.001.

Article Snippet: Cysteine cathepsin inhibitor E-64 , Wako Chemicals , Cat# 331-40963.

Techniques: Infection, Activity Assay, Expressing